The science of our cell processing may not be interesting to everybody, but it is the MOST important reason Bennulife is different from the rest. Our scientists and doctors have been researching cells and performing stem cell procedures for more that 15 years and, because of this, we are at the forefront of the science surrounding stem cell therapies and are always focused on improving our processes to match the current research.
Our doctors work exclusively with Celling Bioscience FDA approved and patented devices, that allow them to very accurately isolate the cells and proteins that will be most healing. This device allows the doctor to recover over 90 percent of the available mesenchymal stem cells (MSCs) from your bone marrow. Then, we can inject them very precisely at the site of the injury. Most other doctors use devices that recover only 30 percent of the MSCs – what a waste to leave all those regenerative cells behind!
The mesenchymal stem cells (MSCs) are the primary cells that we want to retrieve from the bone marrow for effective healing. These incredible cells have the power to do several things:
- Aid in the fight against cell death
- Help reduce inflammation
- Differentiate into other important tissues (bone, cartilage, muscle, fat)
MSCs get all the attention when we talk about regenerative medicine, but there is so much more than just stem cells in the bone marrow we remove from your hip. The growth factors, proteins, and platelets are all equally as important to the healing process!
When we aspirate your bone marrow, the MSCs are mixed in with a variety of other cells. So, we must first isolate the MSCs and growth factors before injecting it into your injured area.
We generally remove between 60 and 120cc of bone marrow from your hip bone in a quick, in-office procedure. Then, we use a centrifuge to spin down the bone marrow aspirate to separate the different cells into a few distinct layers by density.
The three layers are:
- Buffy coat: Here is where we find the MSCs, white blood cells, and platelets.
- Plasma: This includes proteins and growth factors, both of which are important for healing!
- Red blood cells: These are less important for healing, so they are discarded.
The buffy coat is the layer that is most important for healing. Of course, the MSCs—by themselves—can multiply and differentiate into cells to build the tissue that it is healing and can replace, for example, cartilage or bone cells.
But this is only the beginning of the powerful healing potential of MSCs!
MSCs secrete proteins and growth factors that have many trophic effects. They attract circulating cells that aid in healing. The MSCs also produce proteins such as Interleukin-1-Receptor Antagonist Protein (IRAP), a compound that reduces excess inflammation and helps to relieve pain and to restore the homeostasis of an area to provide a more ideal environment for tissues to heal. In all, MSCs secrete approximately 44 different bioactive factors that aid in tissue repair.
Platelets release several types of growth factors including Platelet Derived Growth Factor (PDGF) and Vascular Endothelial Growth Factor (VEGF). These growth factors aid in the healing of diseased or damaged tissue by encouraging new blood vessel growth in the area.
3. White blood cells
White Blood Cells aid the healing process. They have an anti-microbial affect that helps reduce infection in the area where they are injected.
Many doctors will discard the plasma layer because it is simply too much liquid to inject into a tiny joint space. Not us!!
Through our many years of research, we have found that plasma contains many proteins and growth factors that are critical for healing – so why waste them?
We utilize a device that allows us to concentrate these plasma down to less than 5cc so it can be injected into the joint to aid healing in the area of focus.
Some of the important proteins found in the concentrated plasma include:
Alpha 2 Macroglobulin (⍺2M)
- Entraps proteinases to help reduce unwanted tissue destruction and inflammation.
- Binds several cytokines which are inflammatory.
- Slows the breakdown of cartilage surfaces.
Interleukin-1-Receptor Antagonist Protein (IRAP)
- Suppresses the inflammation response.
- There are encouraging clinical trials using recombinant IRAP to treat rheumatoid arthritis.
- IRAP concentrations in bone marrow plasma are 200x greater than they are in peripheral blood plasma
Vascular Endothelial Growth Factor (VEGF)
- Creates new blood vessels after injury.
- Stimulates endothelial cell division and cell migration.
- Anti-inflammatory, anti-apoptotic, anti-scarring, and pro-angiogenic.
Stem cell therapy is a major investment in your longterm health! Processing cells may not seem like an important thing to discuss with your provider, but it may be the difference between optimal pain relief and no healing at all. For the BEST healing outcome, be certain you are receiving the maximum number of MSCs and growth factors!
Would you like to learn if you are a good candidate for a BennuLife stem cell therapy? Schedule a consultation today!